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Visualization of the nucleoid in living bacteria on poly‐lysine coated surfaces by the immersion technique
Author(s) -
Binnerts J. S.,
Woldringh C. L.,
Brakenhoff G. J.
Publication year - 1982
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1982.tb00351.x
Subject(s) - nucleoid , lysine , refractive index , chemistry , immersion (mathematics) , bacteria , bovine serum albumin , reflectivity , biophysics , materials science , biochemistry , escherichia coli , optics , biology , amino acid , optoelectronics , physics , genetics , mathematics , pure mathematics , gene
SUMMARY A method for visualizing the nucleoid in living bacteria is described. The method involves immobilization of the cells by attachment to a poly‐l‐lysine coated slide and subsequent immersion in bovine serum albumin solutions of defined refractive index and osmolality. Dependence of the visibility of the nucleoid on the refractive index of the medium is investigated.