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The use of polystyrene microcarriers to prepare cell monolayers for transmission electron microscopy
Author(s) -
Sargent G. F.,
Sims T. A.,
McNeish A. S.
Publication year - 1981
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1981.tb01260.x
Subject(s) - microcarrier , polystyrene , monolayer , transmission electron microscopy , materials science , microscopy , electron microscope , chemical engineering , nanotechnology , chemistry , cell , optics , polymer , composite material , biochemistry , physics , engineering
SUMMARY A simple method is described, using polystyrene microcarrier beads, that allows cell monolayers to be prepared without distortion for transmission electron microscopy. Cells cultured on polystyrene beads (Biosilon R, Nunc, Kamstrup, Denmark) are held in an agar matrix. Before embedding, the beads are dissolved in 1, 2‐epoxypropane. The technique increases the number of observable cells, when compared to preparations of planar monolayers, and there is good preservation of morphological detail.