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Microdensitometry with image analyser video scanners
Author(s) -
Jarvis L. R.
Publication year - 1981
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1981.tb01229.x
Subject(s) - densitometer , analyser , calibration , computer science , detector , computer vision , glare , artificial intelligence , optics , microscope , optical density , materials science , physics , mathematics , statistics , layer (electronics) , composite material
SUMMARY A numerical method for calibration of the Quantimet 720 1D Auto detector as a densitometer in absolute density units has been developed and applied to integrated microdensitometric measurement. A procedure for the estimation and correction of microscope glare error has also been derived. The error after glare correction was found to be comparable with threshold setting variation encountered in routine use of the Quantimet. The method should prove valuable in densitometric applications not only for Quantimet image analysers but also for any instrument employing a video imaging system because the underlying principles are the same.