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Estimation of surface labelling on whole, critical point dried, cells by microprobe analysis in the scanning electron microscope
Author(s) -
Gwynn I.
Publication year - 1981
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1981.tb01228.x
Subject(s) - electron microprobe , microprobe , materials science , coating , scanning electron microscope , sputtering , electron microscope , microscope , labelling , metal , analytical chemistry (journal) , optics , chemistry , nanotechnology , thin film , mineralogy , composite material , chromatography , metallurgy , physics , biochemistry
SUMMARY A method is described whereby the relative amount of histochemical label, such as ferritin, provided it is exclusively attached to the surface of cells, can be estimated. The X‐ray emission from the metal coating, deposited from a diode‐sputter coating unit, is used to correct for the variations in the iron counts from the label which could occur due to changing analysis conditions.