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A method for determining the three‐dimensional form of active flagella, using two‐colour darkground illumination
Author(s) -
Woolley D. M.
Publication year - 1981
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1981.tb01220.x
Subject(s) - flagellum , object (grammar) , computer vision , artificial intelligence , micrograph , field (mathematics) , optics , biological system , computer science , physics , biology , mathematics , biochemistry , scanning electron microscope , gene , pure mathematics
SUMMARY Using darkground illumination, and placing colour filters over half the field, one can make a single, highly resolved, colour micrograph of a filamentous object (such as a mammalian sperm tail) from which it is possible to get a complete and unambiguous three‐dimensional reconstruction.