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Spatially filtered images of B. subtilis α‐amylase crystals
Author(s) -
Shelley Kirk,
McPherson Alexander
Publication year - 1981
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1981.tb01213.x
Subject(s) - fourier transform , electron microscope , diffraction , crystallography , microscopy , electron micrographs , materials science , chemistry , optics , physics , quantum mechanics
SUMMARY Microcrystals of B. subtilis α‐amylase have been negatively stained and examined by electron microscopy. The micrographs were then subjected to spatial filtering and averaging using a Fourier transform procedure programmed for a mini computer. From these ‘enhanced’ images refined crystallographic parameters were obtained and a model for the packing of the asymmetric units within the crystal was derived. In addition, these parameters obtained from electron microscopy are compared with those derived from limited X‐ray diffraction data on macrocrystals of the same form.