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Preparation of vascular endothelium for scanning electron microscopy: a comparison of the effects of perfusion and immersion fixation
Author(s) -
Gertz S. David,
Rennels Marshall L.,
Forbes Michael S.,
Nelson Erland
Publication year - 1975
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1975.tb04065.x
Subject(s) - scanning electron microscope , perfusion , fixation (population genetics) , endothelium , in situ , immersion (mathematics) , biomedical engineering , pathology , chemistry , anatomy , materials science , medicine , radiology , biochemistry , composite material , organic chemistry , gene , mathematics , pure mathematics
SUMMARY A comparison of currently used methods of tissue fixation for scanning electron microscopic study of vascular endothelium revealed that in situ fixation by intravascular perfusion is superior to immersion for the preservation of endothelial surface morphology.
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