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Cellular responses of leucocytes isolated from the gut of rainbow trout, Oncorhynchus mykiss (Walbaum)
Author(s) -
DAVIDSON G. A.,
ELLIS A. E.,
SECOMBES C. J.
Publication year - 1991
Publication title -
journal of fish diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.819
H-Index - 85
eISSN - 1365-2761
pISSN - 0140-7775
DOI - 10.1111/j.1365-2761.1991.tb00623.x
Subject(s) - rainbow trout , biology , phagocytosis , immune system , chemotaxis , digestion (alchemy) , stimulation , ionophore , calcium , lysozyme , fish <actinopterygii> , microbiology and biotechnology , immunology , biochemistry , endocrinology , fishery , medicine , chemistry , receptor , chromatography , membrane
Abstract. Leucocytes were isolated from the gut of rainbow trout by collagcnase digestion. They were shown to be capable of producing a chemoattractant(s) for head kidney leucocytes and a macrophagc activating factor, following stimulation with calcium ionophore and T‐cell mitogens, respectively. They also included low but significant numbers of phagocytes, as determined by phagocytosis of fluorescent latex microspheres and oxygen free radical production. However, migration of isolated gut cells in response to head kidney chemo‐attractants was lacking. The significance of these results in relation to mucosal immunity is discussed.