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Methods for the recovery of a model virus from healthcare personal protective equipment
Author(s) -
Casanova L.,
Rutala W.A.,
Weber D.J.,
Sobsey M.D.
Publication year - 2009
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.2008.04093.x
Subject(s) - personal protective equipment , infectious dose , virus , infection control , contamination , virology , microbiology and biotechnology , covid-19 , chemistry , medicine , chromatography , biology , infectious disease (medical specialty) , intensive care medicine , disease , ecology , pathology
Abstract Aims: To develop methods for recovering a model virus (bacteriophage MS2) from healthcare personal protective equipment (PPE). Methods and Results: Nine eluents were evaluated for recovery of infectious MS2 from PPE: 1·5% beef extract (BE) pH 7·5 with and without 0·1% Tween 80, 1·5% BE pH 9·0 with and without 0·1% Tween 80, 3% BE pH 7·5 with and without 0·1% Tween 80, 3% BE pH 9·0 with and without 0·1% Tween 80 and PBS with 0·1% Tween 80. Methods were applied to experimentally contaminated PPE. Elution followed by two‐step enrichment assay could recover virus inputs as low as 1·5 log 10 , and could recover >90% of inoculated virus from used items of experimentally contaminated PPE worn by human volunteers. Conclusions: BE was effective for recovering infectious viruses from a range of PPE materials. Significance and Impact of the Study: PPE plays a crucial role in interrupting transmission of infectious agents from patients to healthcare workers (HCWs). The fate of micro‐organisms when PPE is removed and disposed of has important consequences for infection control. Methods described here can be used to conduct rigorous studies of viral survival and transfer on PPE for risk assessments in infection control and HCW protection.