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Expression of novobiocin resistance genes in the novobiocin‐producing organism Streptomyces niveus
Author(s) -
Hoggarth J.H.,
Ritchie D.A.
Publication year - 1995
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1995.tb03097.x
Subject(s) - novobiocin , biology , mutant , streptomyces , gene , streptomycetaceae , microbiology and biotechnology , genetics , actinomycetales , antibiotics , bacteria
RNA isolated at intervals during fermentation from the novobiocin‐producing wild‐type strain of Streptomyces niveus and from a series of novobiocin‐non‐producing (Nov ‐ ) mutants was hybridized to DNA probes containing sequences which specify novobiocin resistance. The probes were made from inserts contained in the clones pGL101 and pGL103 which increase the level of novobiocin resistance of S. lividans transformants from 10 μg ml ‐1 to 50 μg ml ‐1 and 150 μg ml ‐1 , respectively. No hybridization was detected with the pGL101 probe. The pGL103 probe hybridized to RNA extracted during the later stages of growth—a pattern corresponding to the transition from low to high level novobiocin resistance during growth of S. niveus wild‐type cultures. Neither probe hybridized to RNA extracted from four Nov ‐ mutants. These mutants showed variable levels of novobiocin resistance but none expressed the high wild‐type levels. The authors conclude that expression of the DNA sequence in pGL103 is associated with high level novobiocin resistance.