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Two‐dimensional gel resolution of polypeptides specific for autotrophic growth in Thiobacillus versutus
Author(s) -
Read Dorothy L.,
Matin A.
Publication year - 1987
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1987.tb04871.x
Subject(s) - thiobacillus , autotroph , biochemistry , thiobacillus ferrooxidans , chemistry , gel electrophoresis , sulfur , biology , chromatography , microbiology and biotechnology , bacteria , genetics , organic chemistry
Seven polypeptides specific for autotrophic growth by oxidation of thiosulphate have been identified in proteins of Thiobacillus versutus analysed by two‐dimensional gel electrophoresis. Their molecular weights ranged from 32 to 100 kD and PI values from pH 6 to pH 7. Their probable relationships to previously reported Thiobacillus proteins involved in autotrophy, in particular ribulose bis‐phosphate carboxylase, are discussed. These polypeptides will serve as useful landmarks in genetic study and manipulation of sulphur oxidation in these organisms.