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The cytochrome c oxidase test for the rapid detection of psychrotrophic bacteria in milk
Author(s) -
Kroll R.G.
Publication year - 1985
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1985.tb03312.x
Subject(s) - psychrotrophic bacteria , pasteurization , raw milk , food science , bacteria , chemistry , microbiology and biotechnology , biology , genetics
A new, rapid, simple and cheap method for the detection of the predominant psychrotrophic bacteria in raw and pasteurized milks is described, using tetramethyl‐ p ‐phenylene‐diamine dihydrochloride to detect cytochrome c oxidase which in general is not present in the non‐psychrotrophic bacterial milk flora. The test is sensitive to samples containing over 10 4 organisms/ml. Correlation coefficients of 0.92 and 0.84 between dye oxidation and viable counts for pasteurized and raw milk samples, respectively, were found.