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The Sterility Testing of Aminoglycosides: A New Approach Using Enzymic Inactivation
Author(s) -
SIMPSON ANN M.,
BREEZE A. S.
Publication year - 1981
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1981.tb04249.x
Subject(s) - escherichia coli , sterility , enzyme , substrate (aquarium) , biochemistry , chemistry , substrate specificity , biology , genetics , ecology , gene
The inactivation of various aminoglycosides prior to sterility testing has been studied. The preparation of the enzyme used in the inactivation, a 3‐N‐acetyl transferase of wide substrate specificity from Escherichia coli JR 225, is described. The enzyme is partially purified and some of its kinetic parameters described. The extent of the inactivation of the individual aminoglycosides can be related to the physiological efficiency of the enzyme. The lyophilization of the components of the inactivating system is successful when undertaken individually, but not together.