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A Note on the Inhibition of Pseudomonas aeruginosa by a Modification of Brilliant Green Agar for Improved Salmonella Isolation
Author(s) -
WALKER A. P.
Publication year - 1981
Publication title -
journal of applied bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 0021-8847
DOI - 10.1111/j.1365-2672.1981.tb01259.x
Subject(s) - brilliant green , pseudomonas aeruginosa , agar , salmonella , nutrient agar , isolation (microbiology) , microbiology and biotechnology , agar plate , serotype , pseudomonas , biology , strain (injury) , food science , bacteria , chemistry , genetics , organic chemistry , anatomy
A strain of Pseudomonas aeruginosa having colonies that resemble those of salmonellas on brilliant green agar is almost totally inhibited by the addition of 1.0 mg/ml of sulphacetamide to the medium. Low numbers of Ps. aeruginosa grew equally well on brilliant green and nutrient agar, but 10 6 –10 7 organisms were needed before any growth appeared on the medium containing sulphacetamide. During 12 months of routine use of the sulphacetamide medium, involving almost 3000 plates, Ps. aeruginosa has been isolated as a contaminant only once. Forty‐seven salmonella serotypes were grown on the sulphacetamide brilliant green agar in the same period.

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