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Genetic Identification of Lophius budegassa and L. piscatorius by PCR‐RFLP Analysis of a Mitochondrial tRNA Glu /Cytochrome b Segment
Author(s) -
Sanjuan A.,
RaposoGuillán J.,
Comesañta A.S.
Publication year - 2002
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.2002.tb08792.x
Subject(s) - restriction fragment length polymorphism , biology , restriction enzyme , polymerase chain reaction , microbiology and biotechnology , cytochrome b , mitochondrial dna , genetics , restriction site , dna , gene
Identification of Lophius budegassa (black‐bellied angler) and L. piscatorius (angler) (Lophiiformes) was carried out on the amplification of a 486 bp tRNA Glu /cytochrome b segment using the polymerase chain reaction (PCR). Direct DNA sequencing of 6 PCR products was carried out. Six restriction endonucleases ( Alu I, Cfo I, Hae III, Hin fI, Mae , and Scr FI) with different species‐specific restriction fragment length polymorphism (RFLP) were selected. Digestions of PCR products from 30 individuals showed no intraspecific polymorphism. Double digestions ( Cfo I and Hin fI, and Hae III and Scr FI) were simpler and more rapid than single digestions. This technique is suitable for distinguishing tails of both Lophius species.