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Detection of Viable Rotaviruses in Shellfish by means of Cell Culture and Immunofluorescence Assay
Author(s) -
Santos C. S.,
Rigotto C.,
Simoes C.M.O.,
Barardi C.R.M.
Publication year - 2002
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.2002.tb08738.x
Subject(s) - oyster , immunofluorescence , biology , rotavirus , microbiology and biotechnology , virology , shellfish , monoclonal antibody , cell culture , reoviridae , inoculation , lysis , virus , antibody , fishery , fish <actinopterygii> , aquatic animal , immunology , genetics
ABSTRACT: The goal of this work was to examine the use of cell culture and immunofluorescence assays to detect viable rotaviruses in artificially seeded oyster meat and to determine if the method for oyster extracts preparation affects the viability of the viruses. Oyster tissues were seeded with rotavirus SA11, followed by tissue extract preparation. For cytopathogenicity assays (CPE), non‐cytotoxic dilutions of seeded oyster extracts were inoculated in MA 104 cells. For immunofluorescence assays (IFA) the monoclonal antibody Mab M60 anti‐VP7 capsid glycoprotein of rotavirus was used. For CPE tests, no significant difference between the oyster extracts inoculated with rotavirus before and after extract preparation was detected. Similar results have been observed in IFA assays and the recoveries of viable rotavirus obtained were close to 100%.