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Linking Solid Phase Extraction and Enzyme‐Linked Immunosorbent Assay to Measure Chlorpyrifos in Fish Tissue
Author(s) -
Wan P.,
Santerre C.R.,
Deardorff D.C.
Publication year - 2001
Publication title -
journal of food science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.772
H-Index - 150
eISSN - 1750-3841
pISSN - 0022-1147
DOI - 10.1111/j.1365-2621.2001.tb11331.x
Subject(s) - chromatography , solid phase extraction , chemistry , extraction (chemistry) , detection limit , hexane , elution , fish <actinopterygii> , chlorpyrifos , silica gel , correlation coefficient , linear correlation , pesticide , biology , fishery , statistics , mathematics , agronomy
A method for measuring chlorpyrifos in fish tissue coupling solid phase extraction (SPE) and enzyme‐linked immunosorbent assay (ELISA) is described. Fish tissue was added to a silica gel column and extracted with hexane. The eluant was passed through an SPE column (C‐18) and analyzed using ELISA. The method, which was comparable to an FDA GC/ECD method, gave recoveries from 83.4% to 87.5% for fortified fish samples, a lower limit of detection of 0.01 ppm, a linear range from 0.01 to 0.5 ppm, and correlation coefficients from 0.86 to 0.96. Nineteen contaminated fish samples analyzed using both ELISA and GC/ECD methods gave comparable chlorpyrifos concentrations with a correlation coefficient of 0.78.