Determination of β‐Carotene Stereoisomers by Spectrophotometric Analysis | Zendy

EDWARDS C. G. | Zendy; LEE C. Y. | Zendy

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Determination of β‐Carotene Stereoisomers by Spectrophotometric Analysis

Author(s) -

EDWARDS C. G.,

LEE C. Y.

Publication year - 1986

Publication title -

journal of food science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.772

H-Index - 150

eISSN - 1750-3841

pISSN - 0022-1147

DOI - 10.1111/j.1365-2621.1986.tb11177.x

Subject(s) - absorbance , carotene , chemistry , chromatography , spectrophotometry , organic chemistry

A method for determining the amount of β‐carotene stereoisomers using spectrophotometric analysis was developed. Water‐soluble β‐carotene was dissolved in pH 6.0 buffer and heated. The stereoisomers formed were separated by column chromatography and quantified using spectrophotometric analysis. The %D B /D 11 value (absorbance of the cis ‐peak divided by the absorbance at the maximum wavelength) determined for the β‐carotene solutions was found to be linearly correlated to the isomer composition (r = 0.971).

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