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Bioconversion of sugar cane bagasse for cellulase enzyme and microbial protein production
Author(s) -
GARG S. K.,
NEELAKANTAN S.
Publication year - 1982
Publication title -
international journal of food science and technology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.831
H-Index - 96
eISSN - 1365-2621
pISSN - 0950-5423
DOI - 10.1111/j.1365-2621.1982.tb00182.x
Subject(s) - bagasse , cellulase , bioconversion , cellulose , chemistry , food science , fermentation , sugar , incubation , cane , aspergillus terreus , substrate (aquarium) , carboxymethyl cellulose , single cell protein , microbiology and biotechnology , biochemistry , biology , sodium , ecology , organic chemistry
Summary A pilot scale (10 1) fermenter trial was conducted for the production of cellulase enzyme and single cell protein by growing a cellulolytic mould Aspergillus terreus GN 1 on 1.0% alkali‐treated sugar cane bagasse substrate. A maximum crude protein content of 29.8% in the biomass was obtained during 4 days fermentation from the initial alkali‐treated bagasse having 3.0% crude protein content. The carboxymethyl cellulase (CMCase) activity was almost 0 u/ml during the first 24 hr, which increased during 96 hr incubation to 0.52 u/ml, and corresponded with the highest filter paper enzyme (FPase) activity of 0.032 u/ml. There was an alternate decrease and further increase in water soluble carbohydrate during 96 hr incubation, which increased finally to 7.9%. There was no cellulose utilization during the first 24 hr of incubation. However, the cellulose of sugar cane bagasse substrate was utilized subsequently and a maximum of 72.5% of the initial cellulose was utilized up to 96 hr fermentation time.