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Ex vivo assessment of genotoxicity and cytotoxicity in murine fibroblasts exposed to white MTA or white Portland cement with 15% bismuth oxide
Author(s) -
Zeferino E. G.,
Bueno C. E. S,
Oyama L. M.,
Ribeiro D. A.
Publication year - 2010
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/j.1365-2591.2010.01747.x
Subject(s) - portland cement , mineral trioxide aggregate , genotoxicity , cytotoxicity , comet assay , ex vivo , trypan blue , materials science , cement , chemistry , medicine , composite material , dentistry , toxicity , biochemistry , dna damage , apoptosis , in vitro , organic chemistry , dna
Zeferino EG, Bueno CES, Oyama LM, Ribeiro DA.Ex vivo assessment of genotoxicity and cytotoxicity in murine fibroblasts exposed to white MTA or white Portland cement with 15% bismuth oxide. International Endodontic Journal , 43 , 843–848, 2010. Abstract Aims To evaluate whether white mineral trioxide aggregate (MTA) or white Portland cement with 15% bismuth oxide were able to induce genetic damage and cellular death ex vivo. Methodology Aliquots of 1 × 10 4 murine fibroblasts were incubated at 37 °C for 3 h with MTA (white) or white Portland cement with 15% bismuth oxide, at final concentrations ranging from 10 to 1000 μg mL −1 individually. Data of three independent repeats from the comet assay and the trypan blue exclusion test were assessed by the one‐way anova followed by Tukey’s test. Results Mineral trioxide aggregate or Portland cement containing bismuth oxide did not produce genotoxic effects with respect to the single‐cell gel (comet) assay data for all concentrations evaluated. Furthermore, no cytotoxicity was observed for MTA or Portland cement. Conclusion White MTA or white Portland cement containing 15% bismuth oxide were not genotoxic and cytotoxic.