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In vitro genotoxicity of root canal sealers
Author(s) -
Brzovic V.,
Miletic I.,
Zeljezic D.,
Mladinic M.,
Kasuba V.,
Ramic S.,
Anic I.
Publication year - 2009
Publication title -
international endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.988
H-Index - 119
eISSN - 1365-2591
pISSN - 0143-2885
DOI - 10.1111/j.1365-2591.2008.01510.x
Subject(s) - genotoxicity , comet assay , chromosome aberration , microbiology and biotechnology , negative control , dna damage , biology , chemistry , chromosome , dna , medicine , toxicity , traditional medicine , genetics , organic chemistry , gene
Aim To evaluate the effect of leakage on differences in genotoxicity of root canal sealers ex vivo according to their main components using two different cytogenetic assays. Methodology Six materials of different composition (GuttaFlow, Epiphany, Diaket, IRM, SuperEBA and Hermetic) were tested on human peripheral blood lymphocytes using the comet assay and chromosomal aberration analysis. Prepared materials were eluted in physiological solution for 1 h, 1 day, 5 and 30 days. Thereafter cultures were treated with 8 μg, 4 μg and 2 μg of each sealer. Frequencies of chromatide and chromosome breaks and accentric fragments were determined. Comet assay was used to evaluate primary DNA damage by measuring tail length and tail intensity. Chi‐square, Fisher’s PLSD (Protected Least Significant Difference) and Kruskall–Wallis non parametric tests were used for statistical analysis. Results After 1‐h elution only the highest dose of Diaket, Hermetic and SuperEBA significantly ( P = 0.035, P = 0.048, P = 0.037 respectively) affected the measured cytogenetic parameters. The migration ability of DNA was more strongly affected than induction of chromosomal aberrations. After elutions longer than 24 h none of the tested sealers exhibited a genotoxic effect. Conclusion Under the conditions used in the study all sealers had acceptable biocompatibility in terms of genotoxicity.