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Molecular cloning and expression patterns of a putative olfactory diacylglycerol kinase from the noctuid moth Spodoptera littoralis
Author(s) -
Chouquet B.,
Bozzolan F.,
Solvar M.,
Duportets L.,
JacquinJoly E.,
Lucas P.,
Debernard S.
Publication year - 2008
Publication title -
insect molecular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.955
H-Index - 93
eISSN - 1365-2583
pISSN - 0962-1075
DOI - 10.1111/j.1365-2583.2008.00814.x
Subject(s) - spodoptera littoralis , biology , diacylglycerol kinase , spodoptera , molecular cloning , complementary dna , olfactory system , insect , microbiology and biotechnology , signal transduction , lepidoptera genitalia , genetics , botany , protein kinase c , gene , neuroscience , noctuidae , recombinant dna
Abstract In the aim of the characterization of the molecular actors of insect olfactory transduction, we have cloned the full cDNA encoding a Spodoptera littoralis diacylglycerol kinase (DGK) named SlDGK. In male adults, SlDGK transcript was detected predominantly in the brain and in the olfactory sensilla trichodea located on the antennae. SlDGK expression was first detected at day 3 of the pupal stage, then reached a maximum at the end of this stage and was maintained at this level during the adult period. These data provide the first molecular characterization of a DGK potentially involved in the regulation of signalling pathways responsible for the establishment and/or the functioning of the olfactory system in Lepidoptera.