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A DNA adjuvant encoding a fusion protein between anti‐CD3 single‐chain Fv and AIMP1 enhances T helper type 1 cell‐mediated immune responses in antigen‐sensitized mice
Author(s) -
Lee Byeong Cheol,
O’Sullivan Insug,
Kim Eugene,
Park Sang Gyu,
Hwang Seung Yong,
Cho Daeho,
Kim Tae Sung
Publication year - 2009
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1111/j.1365-2567.2008.02880.x
Subject(s) - biology , immune system , antigen , t cell , immunoglobulin class switching , ovalbumin , microbiology and biotechnology , adjuvant , antibody , priming (agriculture) , immunology , b cell , botany , germination
Summary T helper type 1 (Th1) cell‐mediated immune responses contribute to host defences against intracellular pathogen infections and cancer. Previously, we found that aminoacyl tRNA synthetase‐interacting multifunctional protein 1 (AIMP1) activated macrophages and dendritic cells to enhance Th1 responses. Herein, we manipulated this property to improve Th1 immune responses in vivo by constructing a mammalian expression plasmid (pAnti‐CD3sFv/AIMP1) encoding AIMP1 fused to the anti‐CD3 single‐chain Fv (sFv), the smallest unit of the antibody that interacts with the CD3ε region of the T‐cell receptor. Intramuscular injection of ovalbumin (OVA)‐sensitized BALB/c mice with pAnti‐CD3sFv/AIMP1 DNA adjuvant increased the OVA‐specific, interferon‐γ production by their CD4 + T cells and the levels of anti‐OVA immunoglobulin G2a (IgG2a) isotype in their sera. Furthermore, the pAnti‐CD3sFv/AIMP1 DNA adjuvant decreased interleukin‐4 production and anti‐OVA IgE levels in the OVA‐injected mice. Importantly, the pAnti‐CD3sFv/AIMP1 was more efficient than a mixture of pAnti‐CD3sFv and pAIMP1 in inducing OVA‐specific Th1 immune responses and also in inhibiting OVA‐specific Th2 responses during antigen priming. These studies indicated that the pAnti‐CD3sFv/AIMP1 fusion DNA adjuvant enhanced Th1 immune responses in antigen‐sensitized mice.