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Detection of Grapevine A vitivirus in Tunisian grapevines
Author(s) -
M'hirsi S.,
Fattouch S.,
Acheche H.,
Marrakchi M.,
Marzouki N.
Publication year - 2001
Publication title -
eppo bulletin
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.327
H-Index - 36
eISSN - 1365-2338
pISSN - 0250-8052
DOI - 10.1111/j.1365-2338.2001.tb01035.x
Subject(s) - vitis vinifera , biology , horticulture , nepovirus , closterovirus , botany , virology , plant virus , virus
Grapevine A vitivirus (GVA), Grapevine B vitivirus (GVB), Grapevine leaf‐roll associated closterovirus 3 (GLRaV3) and Grapevine fanleaf nepovirus (GFLV) were detected by DAS‐ELISA in samples of grapevine showing symptoms of rugose wood from different viticultural areas of Tunisia. After optimization of experimental conditions, PCR and IC‐RT–PCR were found to be more efficient and sensitive than DAS‐ELISA for the detection of GVA.
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