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Specific identification of Aureobasidium pullulans strain L47 using Scorpion PCR *
Author(s) -
Schena L.,
Sialer M. M. Finetti,
Gallitelli D.
Publication year - 2000
Publication title -
eppo bulletin
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.327
H-Index - 36
eISSN - 1365-2338
pISSN - 0250-8052
DOI - 10.1111/j.1365-2338.2000.tb00947.x
Subject(s) - aureobasidium pullulans , biology , strain (injury) , biological dispersal , colonization , microbiology and biotechnology , food science , population , demography , sociology , fermentation , anatomy
Strain L47 of Aureobasidium pullulans is an effective biocontrol agent for several post‐harvest fruit diseases, even when applied in the field before harvesting. This strategy requires a specific method of monitoring in order to optimize formulations and to evaluate the level of colonization and dispersal in the environment. We have detected strain L47 among other morphologically indistinguishable isolates of A. pullulans using Scorpion PCR, an automated real‐time fluorescence detection system that targets a 145‐bp fragment of the L47 genome. We propose this approach as a specific, rapid and reliable method of monitoring A. pullulans , strain L47.