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The BRAF V600E mutation is not associated with poor prognostic factors in Korean patients with conventional papillary thyroid microcarcinoma
Author(s) -
Kim Tae Yong,
Kim Won Bae,
Song Ja Young,
Rhee Yoon Soo,
Gong Gyungyub,
Cho Yong Mee,
Kim Sang Yoon,
Kim Seong Chul,
Hong Suck Joon,
Shong Young Kee
Publication year - 2005
Publication title -
clinical endocrinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.055
H-Index - 147
eISSN - 1365-2265
pISSN - 0300-0664
DOI - 10.1111/j.1365-2265.2005.02389.x
Subject(s) - thyroid carcinoma , medicine , v600e , mutation , thyroid , follicular cell , carcinoma , pathology , adenoma , cancer research , oncology , gastroenterology , biology , gene , biochemistry
Summary Background The BRAF V600E mutation, the most common genetic alteration reported in papillary thyroid carcinoma, has been associated with poor prognostic factors. Aim To determine whether the presence of the BRAF V600E mutation is associated with poor prognosis in Korean patients with conventional papillary thyroid microcarcinoma (micro‐PTC). Patients and methods DNA was extracted from paraffin‐embedded thyroid tumour specimens taken from 60 patients with conventional micro‐PTC, as well as from nine patients with follicular variant papillary carcinoma, six with nodular hyperplasia, four with follicular carcinoma (including one with Hürthle cell carcinoma), four with follicular adenoma (including two with Hürthle cell adenoma) and one each with medullary carcinoma, poorly differentiated carcinoma and anaplastic carcinoma. The presence of the BRAF V600E mutation was determined by polymerase chain reaction (PCR) amplification of exon 15 followed by direct sequencing. Results The BRAF V600E mutation was detected in tumour samples from 31 of 60 conventional micro‐PTC patients (52%), but was not detected in patients with other types of thyroid tumours. The age distribution, tumour size, extrathyroid extension, multifocality and staging did not differ significantly between patients with and without the BRAF V600E mutation. Conclusion In Korean patients with conventional micro‐PTC, the presence of the BRAF V600E mutation was not significantly associated with prognostic factors.