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17β‐Oestradiol counteracts the formation of the more acidic isoforms of follicle‐stimulating hormone and luteinizing hormone after menopause
Author(s) -
Wide Leif,
Naessén Tord
Publication year - 1994
Publication title -
clinical endocrinology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.055
H-Index - 147
eISSN - 1365-2265
pISSN - 0300-0664
DOI - 10.1111/j.1365-2265.1994.tb02513.x
Subject(s) - luteinizing hormone , endocrinology , medicine , follicle stimulating hormone , menopause , hormone , gene isoform , gonadotropic cell , chemistry , biochemistry , gene
Summary OBJECTIVE When the gonadotropin levels increase at midcycle, more basic isoforms of FSH and LH appear in the circulation. However, when these gonadotrophins increase at menopause more acidic forms appear. The present study was done to see whether chronic 17β‐oestradiol (E 2 ) administration to post‐menopausal women could counteract the formation of the more acidic isoforms after the menopause. DESIGN Serum samples were obtained from 16 postmenopausal women, mean age 70 years (range 63‐84 years), 46‐169 days after the subcutaneous insertion of a 20‐mg E 2 ‐implant. FSH, LH and E 2 in the sera were measured with fluoroimmunoassays. The median charge and the degree of charge heterogeneity of the FSH and LH isoforms were determined for each serum by electrophoresis in 01% agarose suspension. Sera from an age‐matched control group were analysed in parallel. RESULTS The E 2 levels in the E 2 ‐treated women were 230–570 pmoI/I, within the range expected during the midluteal phase of the normal menstrual cycle. The mean serum FSH and LH levels were similar to normal follicular phase FSH and LH levels (8 6 and 20 8% respectively of the control group). It was estimated that individual serum specimens from both groups contained 20–30 different isoforms for both FSH and LH. The median charges of the isoforms of FSH and LH were more basic in all the E 2 ‐treated subjects than in their corresponding untreated controls. The mean median charge for FSH was close to the values for the follicular and luteal phases and that for LH close to that for the luteal phase. In some E 2 ‐treated women the isoforms were even more basic with a charge similar to that at the midcycle peak. The degree of charge heterogeneity for the E 2 ‐treated group was significantly ( P <0.001) larger than for the controls and similar to that during the normal menstrual cycle. CONCLUSION Chronic E 2 administration to post‐menopausal women counteracted the formation of more acidic isoforms of both FSH and LH after the menopause.