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A method for identification of dermatophyte antigens in situ by an immunoperoxidase technique in light and electron microscopy
Author(s) -
HOLDEN C.A.,
HAY R.J.,
MACDONALD D.M.
Publication year - 1981
Publication title -
clinical and experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.587
H-Index - 78
eISSN - 1365-2230
pISSN - 0307-6938
DOI - 10.1111/j.1365-2230.1981.tb02310.x
Subject(s) - dermatopathology , medicine , dermatology
Summary A method, using an immunoperoxidase technique, to demonstrate antigenic sites on dermatophyte hyphae in light and electron microscopy is described. Specific antisera were obtained from rabbits immunized with homogenized whole mycelia. The cell wall is confirmed as a major antigenic determinant and ultrastructurally the location of this antigenicity is shown as a continuous granular deposition of the immunochemical reaction product on the inner and outer aspects of the wall of the fungal hyphae.