Open AccessEffects of enamel matrix proteins on proliferation, differentiation and attachment of human alveolar osteoblasts
Author(s) -
Jiang S.Y.,
Shu R.,
Song Z.C.,
Xie Y.F.
Publication year - 2011
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.2011.00762.x
Subject(s) - bone sialoprotein , osteopontin , osteocalcin , alkaline phosphatase , dental alveolus , enamel matrix derivative , chemistry , cell growth , microbiology and biotechnology , in vitro , matrix (chemical analysis) , regeneration (biology) , osteoblast , enamel paint , viral matrix protein , biology , endocrinology , dentistry , biochemistry , enzyme , medicine , chromatography , gene
Abstract Objectives: Enamel matrix proteins (EMPs) have been demonstrated to promote periodontal regeneration. However, effects of EMPs on human alveolar osteoblasts (hAOBs), up to now, have still been unclear. The purpose of this study was to investigate influence of EMPs on proliferation, differentiation and attachment of hAOBs in vitro . Materials and methods: EMPs were extracted using the acetic acid method, hAOBs were obtained and cultured in vitro . Cell proliferation, alkaline phosphatase (ALP) activity, mRNA expression of osteogenic markers and cell attachment were measured in the absence and in the presence of EMPs (50, 100 and 200 μg/ml). Results: EMPs increased proliferation of hAOBs; however, they inhibited ALP activity and mRNA expression of osteogenic markers (collagen I, ALP, runt‐related protein 2, osteocalcin, bone sialoprotein and osteopontin). Meanwhile, EMPs hindered hAOBs’ attachment. These effects occurred in EMPs concentration‐dependent manner. Conclusions: These results indicate that EMPs may inhibit osteoblastic differentiation and attachment to prevent ankylosis and allow other cell types to regenerate periodontal tissues.