Open AccessRenal transforming growth factor beta 1 production in neonate rats
Author(s) -
Coimbra T. M.,
Silva C. G. A.,
Lachat J. J.
Publication year - 1994
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1994.tb01381.x
Subject(s) - kidney , medicine , transforming growth factor beta , endocrinology , renal cortex , transforming growth factor , immunohistochemistry , medulla , beta (programming language) , messenger rna , cortex (anatomy) , kidney development , biology , gene , biochemistry , neuroscience , computer science , programming language , embryonic stem cell
Abstract. The newborn rat kidney is not fully developed until approximately 12 days after birth. In order to evaluate the possible role of Transforming Growth Factor beta 1 (TGF beta 1) in renal development we analyzed the mRNA level for TGF beta 1 in sixty Wistar rats aged 1,15 and 30 days. We also performed immunohislochemical studies to visualize the distribution of this peptide in the kidney of these rats using a TGF beta 1 antibody. The results show that the mRNA levels for TGF beta 1 are higher in the kidneys of the 1‐day‐old rats than in the 15 (1.4 fold) and 30‐day‐old rats (1.7 fold). The immunohistochemical reaction revealed the presence of TGF beta in the kidneys of the rats. The staining intensity was higher in the renal cortex than in the medulla. The data suggests that TGF beta may be important during kidney development.