Open AccessCell‐Cycle‐Specific Activity of Cyclic Nucleotide Phosphodiesterase In Physarum polycephalum
Author(s) -
Kupetz I. S.,
Jeter J. R.
Publication year - 1985
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1985.tb00645.x
Subject(s) - physarum polycephalum , phosphodiesterase , biochemistry , pde10a , physarum , cyclic nucleotide , enzyme , biology , cyclic nucleotide phosphodiesterase , cell cycle , microbiology and biotechnology , subcellular localization , cell , chemistry , nucleotide , cytoplasm , gene
The cell‐cycle‐related activities of the cAMP‐ and cGMP‐dependent phosphodiesterases of Physarum polycephalum were assayed. the activities of plasmodial homogenate and of selected subcellular fractions were measured. the results suggested the presence of both cAMP‐ and cGMP‐dependent phosphodiesterase in the isolated nuclei of P. polycephalum. In addition, they reveal that the cAMP‐ and cGMP‐dependent phosphodiesterase activities of the subcellular fractions fluctuate throughout the cell cycle. the whole‐cell homogenates exhibit no cell‐cycle‐related changes in the presence of 5 × 10 ‐4 m cGMP. Kinetic data suggest the presence of multiple phosphodiesterase activities in the homogenate and its particulate fractions for the cGMP‐dependent enzyme. Multiple cAMP activities are also suggested for the particulate fractions. the K m values indicate that the substrate affinities of the phosphodiesterases from P. polycephalum are similar to those found previously in mammalian systems.