Open AccessEpidermis Extracts (Chalone) Inhibit Cell Flux At the G 1 ‐S, S‐G 2 and G 2 ‐M Transitions In Mouse Epidermis
Author(s) -
Elgjo K.,
Clausen O. P. F.,
Thorud E.
Publication year - 1981
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1981.tb00507.x
Subject(s) - epidermis (zoology) , hairless , flux (metallurgy) , flow cytometry , colcemid , microbiology and biotechnology , biology , cell cycle , cell growth , cell division , cell , chemistry , biophysics , mitosis , biochemistry , anatomy , organic chemistry
ABSTRACT Epidermal cell flux at the G 1 ‐S, S‐G 2 and G 2 ‐M transition was examined during the first 4 hr after injection of epidermis extract. the flux parameters were estimated by a combination of several methods. the G 1 ‐S and S‐G 2 transit rates were calculated on the basis of a double labelling technique with [ 3 H]TdR, the G 2 ‐M flux by means of colcemid and the relative proportion of cells in the S or G 2 phase by means of flow cytometry. All experiments were performed both in early morning and late evening, corresponding to maximum and minimum rates of epidermal cell proliferation in the hairless mouse. the epidermis extract inhibited the S‐G and G 2 ‐M transit rates to the same degree, while the inhibition of cell flux at the G 1 ‐S transit was consistently stronger. In general, the inhibition of cell flux at the different transitions was most pronounced when the rate of cell proliferation was low and vice versa.