Open AccessLOW LEVEL INCORPORATION OF TRITIATED THYMIDINE INTO THE NUCLEAR DNA OF PURKINJE NEURONS OF ADULT MICE
Author(s) -
Cameron I. L.,
Pool Mary R. H.,
Hoage T. R.
Publication year - 1979
Publication title -
cell proliferation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.647
H-Index - 74
eISSN - 1365-2184
pISSN - 0960-7722
DOI - 10.1111/j.1365-2184.1979.tb00167.x
Subject(s) - thymidine , dna , nuclear dna , biology , dna synthesis , microbiology and biotechnology , chemistry , biochemistry , gene , mitochondrial dna
Adult mice were pulse labeled with tritiated thymidine [ 3 H]TdR and killed 9 hr later. A low level incorporation of [ 3 H]TdR into the nuclear DNA of Purkinje neurons was found in autoradiographs. Enzymatic digestions with DNase and with RNase in combination with autoradiographic grain counts indicate that a portion of nuclear DNA is not stable in the Purkinje nucleus. These results are discussed in light of reports of the stable nature of DNA in Purkinje neurons of adult mice.