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Characteristic expansion of CD45RA + CD27 − CD28 − CCR7 − lymphocytes with stable natural killer (NK) receptor expression in NK‐ and T‐cell type lymphoproliferative disease of granular lymphocytes
Author(s) -
Mitsui Takeki,
Maekawa Izuru,
Yamane Arito,
Ishikawa Tomomi,
Koiso Hiromi,
Yokohama Akihiko,
Handa Hiroshi,
Matsushima Takafumi,
Tsukamoto Norifumi,
Murakami Hirokazu,
Nojima Yoshihisa,
Karasawa Masamitsu
Publication year - 2004
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.2004.05005.x
Subject(s) - lymphoproliferative disease , natural killer cell , microbiology and biotechnology , chemistry , biology , immunology , lymphoma , cytotoxic t cell , biochemistry , in vitro
Summary We analysed the cell surface expression of chemokine receptors and natural killer receptors (NKRs) in addition to conventional T‐ and natural killer (NK)‐cell markers in patients with lymphoproliferative disease of granular lymphocytes (LDGL), and compared results between NK‐ and T‐LDGL subgroups. The subjects of this study were 15 LDGL patients: four NK‐LDGL and 11 T‐LDGL [six CD8 + T‐cell receptor (TCR) α β + , four CD4 + TCR α β + and one CD8 + TCR γ δ + ] cases. Flow cytometric analysis showed that the expanding cells had a common phenotype, CD45RA + CD27 − CD28 − CCR7 − , in NK‐ and T‐LDGL patients irrespective of differences in TCR status. There were no marked differences in the expression patterns of chemokine receptors between NK‐ and T‐LDGL patients. Although restricted NKR subsets were expressed on both NK‐ and T‐large granular lymphocytes (LGLs), CD94 was the most widely expressed marker. These findings may be unique to cells of LDGL cases, because normal CD56 dim NK cells frequently express killer cell immunoglobulin‐like receptors. Furthermore, analysis of NKR expression was repeated over an interval of more than 6 months, and fluctuations of NKR repertoire in the LGL clones were minimal.