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Prevalence of the factor VR506Q mutation in two Irish control populations: use of a novel nested polymerase chain reaction approach
Author(s) -
Livingstone W. J.,
Keenan C.,
White B.,
MynettJohnson L.,
Lawler M.,
Mayne P.,
Smith O. P.
Publication year - 2000
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.2000.02371.x
Subject(s) - nested polymerase chain reaction , polymerase chain reaction , factor v leiden , population , allele , irish , medicine , genetics , allele frequency , virology , biology , demography , environmental health , thrombosis , gene , linguistics , philosophy , venous thrombosis , sociology
The prevalence of factor V (FV) Leiden among normal populations has primarily been determined using blood donors. This control group is carefully selected and therefore may not accurately reflect the true prevalence within the population. We assessed the prevalence of FV Leiden within the Irish population using Guthrie card samples randomly selected from all newborns. We compared this result with the prevalence of FV Leiden within blood donors. A novel nested polymerase chain reaction (PCR) method for FV Leiden was developed for analysis of the Guthrie card samples. There was no significant difference between the allele frequency within the Guthrie card samples and blood donors (2·07% vs. 2·35%, P = 0.66)