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Diverse expression of cytosolic phospholipase A 2 , 5‐lipoxygenase and prostaglandin H synthase 2 in acute pre‐B‐lymphocytic leukaemia cells
Author(s) -
FELTENMARK STINA,
RUNARSSON GHDMUNDUR,
LARSSON PONTUS,
JAKOBSSON PERJOHAN,
BJORKHOLM MAGNUS,
CLAESSON HANSERIK
Publication year - 1995
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1995.tb05588.x
Subject(s) - prostaglandin d2 , arachidonate 5 lipoxygenase , biology , leukotriene , prostaglandin , atp synthase , phospholipase a , lymphoblast , lipoxygenase , gene , microbiology and biotechnology , leukotriene c4 , phospholipase a2 , gene expression , enzyme , cell culture , biochemistry , arachidonic acid , immunology , genetics , asthma
Summary. Several lines of evidence suggest that phospho‐lipases A 2 , leukotrienes and prostaglandins play a role in the proliferation of haemopoietic cells. The expression of genes involved in the biosynthesis of leukotrienes and prostaglandins was investigated in peripheral B lymphoblasts, isolated from eight patients with acute pre‐B‐lymphocytic leukaemia (pre B‐ALL). RT‐PCR analysis demonstrated that four of the investigated pre‐B‐ALL clones expressed the gene coding for cytosolic phospholipase A 2 (cPLA 2 ), but not the gene coding for 5‐lipoxygenase. In contrast, the remaining four pre‐B‐ALL clones expressed 5‐lipoxygenase but not cPLA 2 , suggesting that the transcriptional regulation of these two genes are different and that their cellular functions are not linked to each other. The capacity of pre B‐ALL cells to produce LTB 4 and to express the 5‐lipoxygenase protein, correlated with the expression of 5‐lipoxygenase mRNA. All pre‐B‐ALL clones expressed genes coding for 5‐lipoxygenase activating protein (FLAP), leukotriene A 4 hydrolase and prostaglandin (PG)H synthase 1. Seven of the eight pre B‐ALL clones expressed PGH synthase 2. In comparison, normal tonsillar B cells did not express cPLA 2 or PGH synthase 2.