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The platelet alloantigen Zw a or Pl A1 is expressed by cultured endothelial cells
Author(s) -
Leeksma O. C.,
Giltay J. C.,
ZandbergenSpaargaren J.,
Modderman P. W.,
Van Mourik J. A.,
Kr. von dem Borne A. E. G.
Publication year - 1987
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1987.00355.x-i1
Subject(s) - platelet , glycoprotein , antigen , antibody , endothelial stem cell , microbiology and biotechnology , platelet membrane glycoprotein , biology , membrane glycoproteins , chemistry , biochemistry , immunology , in vitro
Summary Recently, the synthesis by cultured human endothelial cells of a membrane protein complex immunologically related to platelet glycoprotein (GP) IIb/IIIa complex was demonstrated. Since platelet GP IIIa is known to carry the platelet alloantigen Zw a or Pa A1 , studies were performed to establish whether this antigen is also expressed on endothelial cells. The present report describes the results of these studies, which provide evidence for the presence of the Zw a or Pl A1 antigen on the surface of cultured human endothelial cells. This evidence is based on the following observations: (1) cultured endothelial cells react with anti‐Zw a (Pl A1 ) antibodies as shown by indirect immunofluorescence: (2) two proteins are precipitated by anti‐Zw a (Pl A1 ) antibodies from lysates of 125 I‐labelled endothelial cells with an electrophore‐tic mobility corresponding with that of GP IIb and IIIa; (3) anti‐Zw a (Pl A1 ) reacts specifically, as shown by immunoblotting of sodium‐dodecylsulphate polyacrylamide gels of solubilized endothelial cells, with a protein with a mobility similar to that of platelet GP IIIa.