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Intracellular lactic dehydrogenase and phosphohexose isomerase activity in leukaemia and malignant lymphoma
Author(s) -
Ho Anthony D.,
Fiehn Walter,
Hunstein Werner
Publication year - 1982
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1982.tb01964.x
Subject(s) - lactic dehydrogenase , intracellular , medicine , lymphoma , glucose 6 phosphate isomerase , chronic lymphocytic leukemia , myeloid leukaemia , leukemia , myeloid , lymphoblast , immunology , gastroenterology , enzyme , biology , biochemistry , cell culture , genetics
Intracellular activities of total lactic dehydrogenase (LDH) and phosphohexose isomerase (PHI) were investigated in the leukaemic cells of 14 patients with acute myeloid leukaemia (AML), five with chronic myeloid leukaemia (CML), seven with acute lymphoblastic leukaemia (ALL), 19 with chronic lymphocytic leukaemia (CLL), 16 with leukaemic non‐Hodgkin's lymphoma (NHL) and in the lymphocytes of 14 normal persons. Intracellular total LDH‐activity of the blasts of AML and ALL was in the same range as the normal lymphocytes. Patients with CLL and NHL had significantly lower levels ( P <0.01) of total intracellular LDH than the controls. Intracellular PHI activity was consistently lower in the lymphoid malignancies (ALL, CLL, NHL) than in normal lymphocytes ( P <0.05), or in leukaemic myeloblasts ( P <0.01). The intracellular LDH/PHI index of the leukaemic lymphoblasts was significantly elevated as compared to lymphocytes from normal subjects ( P <0.0001) or to leukaemic cells from patients with AML ( P <0.001), with CLL ( P < 0.0001) or with NHL ( P < 0.001). The patients with CLL and NHL, on the other hand, had significantly lower levels of LDH/PHI ratio than the normal subjects ( P <0.0001 and P <0.025 respectively).