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Oxidation of Human and Animal Haemoglobins with Ascorbate, Acetylphenylhydrazine, Nitrite, and Hydrogen Peroxide
Author(s) -
Harvey John W.,
Kaneko J. J.
Publication year - 1976
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1976.tb00922.x
Subject(s) - hydrogen peroxide , nitrite , chemistry , biochemistry , oxidation reduction , ascorbic acid , methemoglobinemia , food science , organic chemistry , nitrate
Partially purified haemoglobin solutions of man, horse, cat and dog were oxidized with ascorbate, acetylphenylhydrazine, nitrite, and H 2 O 2 at 25°C and with ascorbate and acetylphenylhydrazine at 37°C. Haemoglobins of the carnivores were more easily oxidized with ascorbate, nitrite, and H 2 O 2 than equine and human haemoglobins. Feline haemoglobin, in general, appeared more susceptible to oxidation, particularly oxidative denaturation, than those of the other species. In addition, results of the incubations at 37°C suggest that feline haemoglobin B might be more susceptible to oxidative denaturation than feline haemoglobin A. Equine haemoglobins appeared to be the least susceptible to oxidation with ascorbate and nitrite, but were more easily oxidized with acetylphenylhydrazine than those of the dog or man. It is apparent, therefore, that because a haemoglobin is more susceptible to oxidation with one oxidant does not mean that it will be more susceptible to oxidation with all oxidants. Results from the present study support the concept that an oxidant intermediate(s) is more important than H 2 O 2 in drug‐mediated oxidation of haemoglobin.