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Characterization and genomic annotation of polymorphic EST ‐ SSR loci in L itopenaeus vannamei shrimp
Author(s) -
Santos Camilla A,
Rossini Bruno C,
Marques Carla G,
Galetti Pedro M,
Freitas Patrícia D
Publication year - 2012
Publication title -
aquaculture research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.646
H-Index - 89
eISSN - 1365-2109
pISSN - 1355-557X
DOI - 10.1111/j.1365-2109.2011.02956.x
Subject(s) - biology , litopenaeus , shrimp , genetics , locus (genetics) , loss of heterozygosity , expressed sequence tag , allele , microsatellite , gene , genome , fishery
In the present work, EST ‐ SSR (expressed sequence tag‐simple sequence repeat) loci were obtained by screening 45 000 EST s from the P acific white shrimp L itopenaeus vannamei , which was available in a database of the ShEST ( S hrimp EST G enome P roject) consortium. Fifty‐two of 600 EST ‐ SSR loci were selected. From this total, 21 EST ‐ SSR s were polymorphic among 40 individuals and had their gene products ascribed. Two to 20 alleles per locus were detected and the observed heterozygosity ranged from 0.15 to 0.86. Eight loci presented a significant heterozygote deficit after the B onferroni correction, which was attributed to null alleles. Seven loci were able to have their protein products, molecular functions and biological processes determined. Our results are promising for future studies that relate the levels of these gene polymorphisms with different biological responses to stress in aquaculture.