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Effects of polymorphisms in the porcine micro RNA MIR206 / MIR133B cluster on muscle fiber and meat quality traits
Author(s) -
Lee JunSeong,
Kim JunMo,
Lim KyuSang,
Hong JaeSang,
Hong KiChang,
Lee Young Sik
Publication year - 2013
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.2012.02362.x
Subject(s) - biology , single nucleotide polymorphism , snp , genetics , muscle fibre , gene , quantitative trait locus , rna , genotype , allele , candidate gene , anatomy , skeletal muscle
Summary Micro RNA s are a class of small non‐coding RNA molecules that repress gene expression primarily at the post‐transcriptional level. Genetic variations in micro RNA genes may contribute to phenotypic differences by altering the expression of micro RNA s and their targets. Here, we identified 12 single nucleotide polymorphisms ( SNP s) in the genomic region of the porcine MIR206 / MIR133B cluster, 10 and 2 of which were associated with MIR206 and MIR133B respectively. All 12 SNP s were located within primary micro RNA s. Allele frequency determination in different pig breeds ( B erkshire, n = 153; L andrace, n = 125; Y orkshire, n = 173) and association studies of muscle fiber characteristics, lean meat production and meat quality traits were performed on the MIR206 and MIR133B SNP s. The MIR206 SNP s were associated with the percentage of type II a and II b fibers for muscle fiber area composition, meat quality traits including drip loss and lightness, and backfat thickness, a parameter of lean meat production. In addition, we found significant association of the MIR133B SNP s with total muscle fiber number, loin eye area, and muscle p H . Furthermore, these SNP s significantly affected the levels of mature MIR206 and MIR133B , respectively, primarily by regulating the processing of primary micro RNA s into precursor micro RNA s. Interestingly, altered MIR206 levels correlated with phenotypic variability among genotypes of the MIR206 SNP . Our data suggest that polymorphisms in the porcine MIR206 / MIR133B cluster are a genetic factor affecting muscle and meat quality traits.