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Association analyses of single nucleotide polymorphisms in bovine stearoyl‐CoA desaturase and fatty acid synthase genes with fatty acid composition in commercial cross‐bred beef steers
Author(s) -
Li C.,
Aldai N.,
Vinsky M.,
Dugan M. E. R.,
McAllister T. A.
Publication year - 2012
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.2011.02217.x
Subject(s) - fatty acid synthase , polyunsaturated fatty acid , single nucleotide polymorphism , snp , fatty acid desaturase , fatty acid , biology , stearoyl coa desaturase , genotype , food science , gene , biochemistry , gene expression
Summary Two previously reported non‐synonymous coding single nucleotide polymorphisms (SNPs) of bovine stearoyl‐CoA desaturase (delta‐9‐desaturase) ( SCD ) (c.878C>T) and fatty acid synthase ( FASN ) (g:17924A>G) were assessed for their associations with 72 individual and 12 groups of fatty acids in brisket adipose tissue of 223 Canadian commercial cross‐bred beef steers. It was found that the ‘CC’ genotype of the SCD SNP was significantly associated with lower concentrations of saturated fatty acids (SFA) including 10:0, 14:0 and 20:0, higher concentrations of monounsaturated fatty acids including 9 c ‐14:1, 12 c ‐16:1 and 13 c ‐18:1, higher concentrations of polyunsaturated fatty acids (PUFA) including 9 c, 15 c ‐18:2, 10 c, 12 c ‐18:2, 11 c, 13 t ‐18:2 and 12 c, 14 t ‐18:2, but lower concentrations of other PUFA of 9 c, 13 t /8 t, 12 c and 20:2n‐6 ( P < 0.05). The ‘AA’ genotype of the FASN SNP was significantly associated with higher concentrations of SFAs of 10:0, 12:0, 13:0, 14:0 and 15:0, lower concentrations of unsaturated fatty acids of 9 c ‐18:1 and 20:3n‐6, and higher concentrations of unsaturated fatty acids of 9 c ‐14:1 and 12 c ‐16:1 ( P < 0.05). Significant epistatic effects between the SCD and FASN SNP genotypes were also found for several fatty acids including 10:0, 23:0, 6 t /7 t /8 t ‐18:1, 12 t ‐18:1, 13 t /14 t ‐18:1, 16 t ‐18:1, total trans 18:1 and 9 c ,13 t /8 t ,12 c ‐18:2 ( P < 0.05). These results further suggest that SCD and FASN are strong candidate genes influencing fatty acid composition in beef cattle.