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Characterization of a novel microdeletion polymorphism on BTA5 in cattle
Author(s) -
Liu G. E.,
Li R. W.,
Sonstegard T. S.,
Matukumalli L. K.,
Silva M. V. G. B.,
Van Tassell C. P.
Publication year - 2008
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.2008.01769.x
Subject(s) - biology , genotyping , breakpoint , genetics , population , copy number variation , allele , gene , genotype , genome , chromosomal translocation , demography , sociology
Summary We present a detailed breakpoint mapping and population frequency analysis of a 214‐kb microdeletion that removes multiple olfactory receptor genes. Using progressive rounds of PCR assays, we mapped the upstream and downstream breakpoints of this microdeletion event to ∼1 and 12 kb genomic regions, respectively. We developed PCR‐based genotyping assays, characterized a dairy cattle panel of 96 samples and found that the frequency of the deletion allele was over 51%. Our results indicated that this microdeletion is an ancient event occurring in one of the earlier founders, and that it has been stably inherited across generations in the North American dairy cattle population.