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Fine‐mapping of a marbling trait to a 2.9‐cM region on bovine chromosome 7 in Japanese Black cattle
Author(s) -
Hirano T.,
Watanabe T.,
Inoue K.,
Sugimoto Y.
Publication year - 2008
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.2007.01676.x
Subject(s) - marbled meat , quantitative trait locus , biology , genetics , haplotype , chromosome , population , contig , microsatellite , allele , gene , genome , demography , sociology
Summary To locate quantitative trait loci (QTL) for intramuscular fat deposition (marbling) in a local population of Japanese Black cattle, we performed a genome scan using a paternal half‐sib family of Bull A. A marbling QTL was mapped in the region flanked by DIK0079 (20.7 cM) and TGLA303 (39.3 cM) on bovine chromosome (BTA) 7, affecting 5.0% of the total family variance. Haplotype analysis of the QTL region revealed that the marbling‐increasing Q allele was transmitted from the dam. On the other hand, Bull B, a maternal half‐sib of Bull A, did not receive the Q allele from its dam, based on the following findings: (i) a marbling QTL on BTA7 was not detected in the Bull B paternal half‐sib family; (ii) recombination between DIK0079 (20.7 cM) and RM006 (25.4 cM) in the QTL region was observed in the maternal chromosome of Bull B; and (iii) the Q ‐harbouring steers from Bull A exhibited significantly higher marbling than the steers from Bull B and the remaining steers from Bull A. To precisely compare the maternal chromosomes of both bulls, we constructed a bacterial artificial chromosome contig covering the region between DIK0079 and RM006 and developed DNA markers. The recombination occurred between DIK8042 and DIK8044 , indicating that the marbling QTL was in a 2.9‐cM region flanked by DIK0079 and DIK8044 .