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Association of MT‐ND5 gene variation with mitochondrial respiratory control ratio and NADH dehydrogenase activity in Tibet chicken embryos
Author(s) -
Bao H. G.,
Zhao C. J.,
Li J. Y.,
Wu Ch.
Publication year - 2007
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.2007.01622.x
Subject(s) - biology , nadh dehydrogenase , missense mutation , dehydrogenase , genotype , allele , microbiology and biotechnology , gene , mitochondrion , respiratory chain , protein subunit , genetics , biochemistry , enzyme , mutation
Summary NADH dehydrogenase (complex I) couples the oxidation of NADH for the reduction of ubiquinone with the generation of a proton gradient that can be used for the synthesis of ATP. We have found a missense mutation in the MT‐ND5 subunit of NADH dehydrogenase in the Tibet chicken breed. In the present study, the mitochondrial respiratory control ratio (RCR) and NADH dehydrogenase activity in Tibet chicken embryonic brain with different genotypes were measured. Significant differences between animals carrying mitochondria with the EF493865.1:m.1627A vs. EF493865.1:m.1627C alleles were observed for RCR and enzyme activity.