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Sequence analysis of 0.4 megabases of the rabbit germline immunoglobulin kappa1 light chain locus
Author(s) -
Ros F.,
Reichenberger N.,
Dragicevic T.,
Van Schooten W. C. A.,
Buelow R.,
Platzer J.
Publication year - 2005
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.2004.01221.x
Subject(s) - biology , allotype , locus (genetics) , genetics , germline , bacterial artificial chromosome , immunoglobulin light chain , microbiology and biotechnology , antibody , gene , genome
Summary Two rabbit germline bacterial artificial chromosome (BAC) libraries from animals with the b5 and b4 allotype were screened with probes specific for the immunoglobulin kappa1 light chain locus. Two partially overlapping BAC clones containing V κ elements of b5 allotype were isolated from the b5 library and one BAC clone containing J κ 1, C κ and V κ was isolated from the b4 library. These three BAC clones were sequenced. They span about 0.4 MB of the rabbit Ig κ 1 light chain locus including 36 V κ elements, five J elements and the coding region of C κ 1. The organization of the locus and the potential function of newly identified functional and structural elements are discussed.