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A single gel for determining genetic variants of equine erythrocyte carbonic anhydrase ( CA ) and catalase ( Cat )
Author(s) -
BOWLING A. T.,
GORDON L.,
PENEDO M. C. T.,
WICTUM E.,
BEEBOUT J.
Publication year - 1990
Publication title -
animal genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0268-9146
DOI - 10.1111/j.1365-2052.1990.tb03224.x
Subject(s) - carbonic anhydrase , biology , allele , population , red cell , catalase , allele frequency , carbonic anhydrase ii , agarose , genetics , zoology , enzyme , microbiology and biotechnology , biochemistry , gene , medicine , demography , sociology
Summary We describe a method for agarose IEF under acid conditions in which a single gel can be used to diagnose from equine red cell lysates genetic variants for carbonic anhydrase ( CA ) and catalase ( Cat ) Family and population data for 4801 horses of 27 breeds and seven trap sites of Great Basin feral horses are presented to support the presence of a sixth CA allele, CA E , which has been recognized previously, but not described by published data. Allelic frequencies for the two systems suggest it may be appropriate to use this gel for parentage verification programmes or to obtain population data for studies of the genus Equus .