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Procedure for simultaneous phenotyping of genetic variants in cow's milk by isoelectric focusing *
Author(s) -
Seibert Brigitta,
Erhardt G.,
Senft B.
Publication year - 1985
Publication title -
animal blood groups and biochemical genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0003-3480
DOI - 10.1111/j.1365-2052.1985.tb01469.x
Subject(s) - isoelectric focusing , chromatography , separation (statistics) , polyacrylamide , polyacrylamide gel electrophoresis , resolution (logic) , cow milk , biology , genetic variants , chemistry , genetics , biochemistry , microbiology and biotechnology , computer science , food science , artificial intelligence , gene , machine learning , genotype , enzyme
Summary A method is described which allows the simultaneous separation of all polymorphic protein fractions in cow's milk in one single run. The separation could be achieved by isoelectric focusing in ultrathin‐layer polyacrylamide gels. The method is especially suitable for screening purposes because it combines low costs, high resolution and short separation time.