Premium
The inactivation of the bovine J blood group substance by the periodate ion
Author(s) -
Oulevey Jacques,
Thiele Otto Wolfgang
Publication year - 1982
Publication title -
animal blood groups and biochemical genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0003-3480
DOI - 10.1111/j.1365-2052.1982.tb01582.x
Subject(s) - periodate , sialic acid , stroma , biochemistry , sodium periodate , chemistry , periodic acid , biology , chromatography , immunology , organic chemistry , immunohistochemistry
Summary1 Treatment of J‐positive (JR) bovine erythrocytes with periodate (0.25 mmol/1 final concentration, 1 hour, room temperature) has no effect on the J activity. Higher periodate concentrations cause spontaneous haemolyses. 2 Treatment of the lipids extracted from (and containing all J activity of) J cs erythrocytes with periodate leads to a decrease of J activity even with lower periodate concentrations. 3 Treatment of the stroma prepared from J cs erythrocytes with periodate demonstrated the relative stability of the J antigen up to 0.25 mmol/l periodate. At the same time the sialic acid concentration of stroma is reduced to about 13 % of the initial concentration. 4 Desialylation of J cs erythrocytes or J cs stroma with sialidase does not affect the J activity thus confirming previous findings. On the other hand, the J activity of desialylated J cs stroma is much more susceptible to periodate. 5 It is concluded that membrane‐bound sialic acid shields the membrane‐bound J antigen from being attacked by periodate.