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Naturally occurring antibody in bovine fetal serum: reactivity against homologous and heterologous species erythrocytes *
Author(s) -
Miller W. J.,
Hubbert W. T.
Publication year - 1972
Publication title -
animal blood groups and biochemical genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.756
H-Index - 81
eISSN - 1365-2052
pISSN - 0003-3480
DOI - 10.1111/j.1365-2052.1972.tb01228.x
Subject(s) - fetus , antibody , heterologous , biology , agglutinin , homologous chromosome , fetal bovine serum , isoantibodies , agglutination (biology) , immunology , hemagglutination , bovine serum albumin , microbiology and biotechnology , andrology , pregnancy , biochemistry , lectin , in vitro , medicine , genetics , gene
Summary Agglutinins that reacted with red cells from members of a different species (hetero‐agglutinins) were detected in the serum of bovine fetuses beginning with about 5% in the fifth month of gestation, with the frequency of reactive sera increasing to about 50 % near term. The sera were able to agglutinate red cells from an increasing number of species as fetal age increased, but only exceptionally equalled the number agglutinated by sera from the dams. Antibodies that reacted with bovine red cells (isoantibodies) were detected in bovine fetal sera (anti‐L agglutinin, anti‐K hemolysin). However, no fetal serum was found that agglutinated the red cells of the respective dam. All fetal isoantibodies were inactivated by heat (56°C/30 min) and 2‐mercapto‐ethanol (2‐ME) treatment, whereas some heteroagglutinins in serum from one fetus were resistant to inactivation by these methods. This suggests that most of the fetal antibodies were other than IgG.